Dansyl-L-Alanine Cyclohexylammonium Salt (CAS 53332-27-7) is a highly sensitive fluorescent amino acid derivative widely used in proteomics, peptide sequencing, and biochemical analysis. With a molecular formula of C₂₁H₃₁N₃O₄S and a molecular weight of 421.55, this compound is a sulfonamide derivative of L-alanine linked to the well-known 5-(dimethylamino)naphthalene-1-sulfonyl (Dansyl) fluorophore.
As a member of the dansyl amino acid family, it exhibits strong fluorescence and high environmental sensitivity, making it an important reagent for protein structure analysis and molecular interaction studies.
Finetech Industry Limited supplies high-purity Dansyl-L-Alanine Cyclohexylammonium Salt, rigorously tested and suitable for advanced biochemical research, analytical chemistry, and pharmaceutical development.
The analytical power of Dansyl-L-Alanine Cyclohexylammonium Salt originates from the Dansyl chromophore, one of the most widely used fluorescent labeling groups in biochemical research.
The compound typically exhibits:
These properties enable sensitive detection in HPLC fluorescence detectors, fluorescence spectroscopy, and protein labeling experiments.
The fluorescence emission of the Dansyl group is highly dependent on the polarity of the surrounding microenvironment. Changes in solvent polarity or protein binding environments can cause measurable shifts in fluorescence intensity and wavelength.
Because of this solvatochromic property, Dansyl-L-Alanine is widely used to study:
Dansyl-L-Alanine Cyclohexylammonium Salt plays an important role as a reference standard in the Dansyl labeling method for N-terminal amino acid analysis.
The classic Dansyl method, first described by Gray and Hartley (1963), offers approximately 100-fold higher sensitivity compared with the traditional DNP (dinitrophenyl) method.
In analytical workflows, the compound is commonly used in:
These techniques allow researchers to identify N-terminal alanine residues in hydrolyzed peptide fragments with high sensitivity.
In structural biology and protein dynamics research, Dansyl derivatives are frequently used as fluorescent donors or acceptors in FRET systems.
Research published in The Journal of Biological Chemistry highlights the application of Dansyl-labeled amino acids for measuring inter-molecular distances within enzyme-substrate complexes.
FRET studies using Dansyl probes help researchers understand:
The cyclohexylammonium salt form improves the solubility and stability of Dansyl-L-Alanine derivatives.
Studies reported in Analytica Chimica Acta demonstrate the use of dansylated amino acids in developing chiral stationary phases for chromatographic separation.
These systems are widely used for:
Such techniques are essential for ensuring optical purity in L-alanine-derived drugs and biologically active compounds.
| Property | Specification |
|---|---|
| Product Name | Dansyl-L-Alanine Cyclohexylammonium Salt |
| CAS Number | 53332-27-7 |
| Molecular Formula | C₂₁H₃₁N₃O₄S |
| Molecular Weight | 421.55 |
| Purity (HPLC) | ≥ 98.0% |
| Appearance | Light green powder |
| MDL Number | MFCD00038927 |
At Finetech Industry Limited, each batch of Dansyl-L-Alanine Cyclohexylammonium Salt undergoes comprehensive analytical testing to ensure high purity and reliability for research applications.
Available documentation includes:
These documents support high-precision biochemical research and regulatory compliance.
Finetech Industry Limited is a trusted supplier of high-purity biochemical reagents and fluorescent probes for research laboratories worldwide.
Our advantages include:
Our Dansyl-L-Alanine Cyclohexylammonium Salt (CAS 53332-27-7) is ideal for fluorescence labeling, peptide sequencing, protein interaction studies, and advanced analytical techniques.
Gray, W. R., & Hartley, B. S. (1963). A fluorescent method for the determination of the N-terminal residues of peptides. Biochemical Journal.
Weber, G. (1952). Polarization of the fluorescence of macromolecules. Biochemical Journal.
Kinoshita, T., et al. (1974). Fluorescence analysis of amino acids and peptides. Analytical Biochemistry.